Lunaphore COMET

lunaphore_comet_tonsil
Lunaphore website
Technical Brochure
Workflow

The Lunaphore COMET platform is an advanced, fully automated system that integrates liquid handling with high-resolution microscope imaging for sequential, multiplex immunofluorescence staining. Designed for use with standard glass slides and off-the-shelf antibodies, the COMET enables up to 20 distinct staining conditions in a single run, including mono-stains and dual-plexes. Its microfluidic technology ensures minimal reagent use and optimized biochemistry. With a four-slide capacity, the system can achieve up to 40-plex imaging per cycle. Post-run, slides remain compatible for further COMET processing or downstream applications like H&E, sequencing, or in situ hybridization, due to its gentle elution buffer and low-temperature protocol.

Key Features:

  • Adaptable panel development using unlabeled antibodies: Researchers can leverage readily available, off-the-shelf primary antibodies without the need for conjugation or barcoding, enabling rapid assay setup and flexibility in experimental design.
  • Advanced multiplexing: The COMET system enables highly multiplexed spatial analysis, allowing for the simultaneous detection of up to 40 distinct markers on a single tissue slide in one fully automated run.
  • Multiplex staining enables the creation of a cell-by-cell map to decipher complex interactions: The detection and quantification of multiple proteins allows researchers to study not only morphological changes due to pathological or therapeutic conditions, but also to characterize phenotypes at single cell level, and examine cell distance as well as measure cell density in a specific area.
  • Streamlined imaging and analysis: The COMET combines an automated scanner with a high-resolution microscope to deliver subcellular detail. It outputs data in OME-TIFF format that is compatible with leading spatial biology analysis platforms like HORIZON, QuPath, and HALO.